What is HipSci?

The Human Induced Pluripotent Stem Cells Initiative (HipSci) is generating a large, high-quality reference panel of human IPSC lines.

HipSci stands apart from other IPSC projects in a number or respects:

  • Its magnitude – HipSci is systematcially generating IPSCs from hundreds of donors using a standardised experimental pipeline.
  • Cell line availability – HipSci’s cell lines are intended as a national IPSC resource. The cell lines and their accompanying genetic and phenotypic data are free available for use by the wider research community.
  • Disease cohorts – HipSci’s reference panel comprises hundreds of cell lines from phenotypically normal donors, plus several cohorts of donors with inherited genetic diseases.
  • The data – Each line generated is extensively characterised. Assays include genotyping by array, expression array, methylation array, RNA-seq, Exome-seq, proteomics mass spectrometry, and high content cellular phenotyping.
mesoderm differentiation

Induced Pluripotent Stem Cells

Reprogramming of somatic human cells to a pluripotent state was first reported in 2008. In the original study, pluripotency was induced by ectopic expression of four specific transcription factors (OCT4, SOX2, KLF4, and MYC; also known as the Yamanaka factors), which trigger widespread changes in the epigenomes and transcriptional programs of the target cells and ultimately permanently reset their cellular state. Such cells are called induced pluripotent stem cells (iPSC).

Cell lines and data browser