HipSci cell lines undergo thorough characterisation as part of the derivation pipeline. We profile the cell lines using genomics, epigenomics, transcriptomics, proteomics, and high content cellular phenotyping techniques.
How we use the assay data
Assessing pluripotency – Gene expression array data are used to validate pluripotency of derived IPSCs. Furthermore, each IPSC line is differentiated into the three embryonic germ layers and profiled for relevant markers to verify successful differentiation.
Assessing genomic integrity – Aneuploidy and subchromosomal aberrations are known to occur in IPSC derivation. HipSci’s QC procedure uses genotyping array data to detect differences of copy number variation between derived IPS cells and the somatic cells from which they were derived.
Characterisation of the genomic/epi-genomic state of the pluripotent phenotype – HipSci’s extensive panel of IPSC lines allows for great sensitivity in assessing the correlation between transcriptional variation and inter-individual genetic variability. Stem cell biology will benefit from a throrough analysis of the genetic and epigenetic variance of human iPSC, and how these affect phenotype.