The cellular phenotyping project at KCL is evaluating how iPSCs respond to chemical, physical and biological stimuli. A high-content platform has been developed using novel assays and artificial stimuli to analyse iPSC behaviour in different microenvironments. These data contribute to HipSci’s research into the dependence of phenotypic variance on genetic and epigenetic variance.
Undifferentiated iPSCs are enzymatically dissociated and seeded as single cells onto 96-well plates. They are coated with different ECM proteins including fibronectin, collagen, and vitronectin. The plates are stained and then imaged using an Operetta high content device.
This method allows assessment of cell number, proliferation, cell morphology and intercellular adhesion properties.
The results of the cellular phenotyping experiments are distributed as pdf files on the HipSci FTP site.
Complete lists of cellular phenotyping data can be found under the files tab of the cell lines and data browser.