Since initiation of the project, HipSci has developed from scratch a pipeline to derive, expand and bank iPSC lines on a production scale.

iPSC lines are produced in the Cellular Genetics and Phenotyping facility (CGaP) at the Sanger Institute, which opened in February 2013 with HipSci as its initial primary project.

Two candidate lines per donor are picked after reprogramming, from which one is selected based on quality control (QC) assays. For cell lines derived during the earliest phases of the project, three lines per donor were picked as candidates, and in some cases two of these were selected for banking.

HipSci is currently selecting 10 lines per week for expansion, experimental assays and banking. This throughput will generate approximately 400 cell lines by the end of 2015.

The HipSci iPS derivation pipeline